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cPFKL3.0
cPFKL3.0
規(guī)格:
價格:
編號:B194397
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
cPFKL3.0
商品貨號
B194397
Designations
cPFKL3.0
GenBank Number

X15573

培養(yǎng)基
Species
Homo sapiens, human
Depositors
Y Groner
Vector
Construct size (kb): 6.0
Insert
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: liver
Gene product: phosphofructokinase, liver type( human liver-type 1-phosphofructokinase, Phosphofructokinase, liver type) [PFKL]
Alleles: A1, A2
Insert Size (kb)
3.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0 (doublet); BamHI--3.0, 2.0, 1.0; SalI--6.0; XhoI/PstI--3.0, 1.8, 0.55 (doublet); XbaI--3.0 (doublet).
This was constructed from 3 overlapping cDNAs. When transcribed and translated in vitro, a polypeptide of 80 kDa recognized by anti-PFKL antibodies is generated.
The clone detects the following genomic restriction fragments (kb), with those not from PFKL indicated by a '*': BamHI--8.6, 7.5, 5.0, 4.2*, 3.9*, 3.3, 2.9, 2.2*; KpnI--14, 11, 4.2, 3.3*, 2.6; HindIII-- >20, 12.3, 5.2.
A fragment specific to PFKL can be generated from 3' sequences.
The insert contains the complete coding sequences, with 5' and 3' untranslated sequences.
The KpnI polymorphic site is located in the 3.3 kb BamHI genomic fragment .
References

Elson A, et al. The structure of the human liver-type phosphofructokinase gene. Genomics 7: 47-56, 1990. PubMed: 2139864

Levanon D, et al. The primary structure of human liver type phosphofructokinase and its comparison with other types of PFK. DNA 8: 733-743, 1989. PubMed: 2533063

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