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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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cPFKL3.0
cPFKL3.0
規格:
價格:
編號:B242437
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
cPFKL3.0
商品貨號
B242437
Designations
cPFKL3.0
GenBank Number

X15573

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 6.0
Insert
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: liver
Gene product: phosphofructokinase, liver type( human liver-type 1-phosphofructokinase, Phosphofructokinase, liver type) [PFKL]
Alleles: A1, A2
Insert Size (kb)
3.0
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0 (doublet); BamHI--3.0, 2.0, 1.0; SalI--6.0; XhoI/PstI--3.0, 1.8, 0.55 (doublet); XbaI--3.0 (doublet).
This was constructed from 3 overlapping cDNAs. When transcribed and translated in vitro, a polypeptide of 80 kDa recognized by anti-PFKL antibodies is generated.
The clone detects the following genomic restriction fragments (kb), with those not from PFKL indicated by a '*': BamHI--8.6, 7.5, 5.0, 4.2*, 3.9*, 3.3, 2.9, 2.2*; KpnI--14, 11, 4.2, 3.3*, 2.6; HindIII-- >20, 12.3, 5.2.
A fragment specific to PFKL can be generated from 3' sequences.
The insert contains the complete coding sequences, with 5' and 3' untranslated sequences.
The KpnI polymorphic site is located in the 3.3 kb BamHI genomic fragment .
References

Elson A, et al. The structure of the human liver-type phosphofructokinase gene. Genomics 7: 47-56, 1990. PubMed: 2139864

Levanon D, et al. The primary structure of human liver type phosphofructokinase and its comparison with other types of PFK. DNA 8: 733-743, 1989. PubMed: 2533063

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