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Galpha z-WT [pBN36]
Galpha z-WT [pBN36]
規格:
貨期:
編號:B194509
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Galpha z-WT [pBN36]
商品貨號
B194509
Designations
Galpha z-WT [pBN36]
GenBank Number

J03773

Species
Rattus norvegicus, rat
Depositors
P Herzmark, HR Bourne
Applications
in another host, produces protein guanine nucleotide binding protein (G protein), alpha z polypeptide guanine nucleotide-binding protein, alpha z polypeptide
Vector
Construct size (kb): 5.53000020980835
Insert
DNA: cDNA
Insert lengths(kb): 1.529000043869019
Gene product: guanine nucleotide binding protein (G protein), alpha z polypeptide(guanine nucleotide-binding protein, alpha z polypeptide) [GNAZ]
Target Gene: guanine nucleotide binding protein (G protein), alpha z polypeptide
Insert Size (kb)
1.529
Media
ATCC® Medium 1890: LB medium (ATCC medium 1065) with 12 mcg/ml tetracycline and 100 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.8, 0.75; BglI--1.75 (doublet), 1.7, 0.3 + smaller; EcoRI--4.0, 1.55; HindIII--4.7, 0,85; XbaI--5.6.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.65; BglI--1.22, 1.49; BglII--0.97; HindIII--0.71; NcoI--0.01; PvuII--1.52; SacI--0.69; SmaI--0.42; StuI--0.20, 0.43.
Deposited with the understanding that the DNAs not be used in human experiments.
Encodes a hemagglutinin epitope replacing nt 409-426 of the GenBank sequence.
References

Wong YH, et al. Gz-mediated hormonal inhibition of cyclic AMP accumulation. Science 255: 339-342, 1992. PubMed: 1347957

Paul Herzmark, personal communication

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