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Galpha i2 [alpha i2-WT (mouse), pBN31]
Galpha i2 [alpha i2-WT (mouse), pBN31]
規(guī)格:
貨期:
編號:B235148
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Galpha i2 [alpha i2-WT (mouse), pBN31]
商品貨號
B235148
Designations
Galpha i2 [alpha i2-WT (mouse), pBN31]
GenBank Number

M13963

Species
Mus musculus, mouse
Depositors
P Herzmark, HR Bourne
Applications
in another host, produces protein guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Vector
Construct size (kb): 5.360000133514404
Insert
DNA: cDNA
Insert lengths(kb): 1.365000009536743
Gene product: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 [Gnai2]
Target Gene: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Insert Size (kb)
1.365
Media
ATCC® Medium 1890: LB medium (ATCC medium 1065) with 12 mcg/ml tetracycline and 100 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 0.8; BglI--3.1, 1.8, 0.55; BglII--5.35; EcoRI--4.0, 1.4, HindIII--5.35.
The insert contains the following restrictions sites (approximate kb from the 5' end): BamHI--0.73; BglI--0.08; BglII--1.05; BstXI--0.26; EcoRI--1.34; PstI--0.37, 0.43; PvuII--0.41; SacI--0.94; SphI--0.82.
Deposited with the understanding that the DNAs not be used in human experiments.
Restriction site(s) at the 3' end of the insert for linearizing the construct: EcoRV, XhoI.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Wong YH, et al. Mutant alpha subunits of Gi2 inhibit cyclic AMP accumulation. Nature 351: 63-65, 1991. PubMed: 1851251

Paul Herzmark, personal communication

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