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pVNTR-B
pVNTR-B
規格:
價格:
編號:B194966
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pVNTR-B
商品貨號
B194966
Designations
pVNTR-B
Species
Homo sapiens, human
培養基
Depositors
J Groffen
Applications
A 0.6 kb TaqI fragment has been used to detect the VNTR polymorphism.
Vector
Construct size (kb): 6.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 4.199999809265137
Gene product: active BCR-related gene [ABR]
Insert Size (kb)
4.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4, 2.6; EcoRI--7.0; HindIII--7.0; BamHI--7.0; PstI--6.6, 0.4.
The insert is the 4.2 kb HindIII/EcoRI fragment containing VTR B and exons 4 and 5. The repeat elements are in the HindIII/XhoI fragment of about 1.8 kb.
The exons are close to the EcoRI end, with exon 5 contained in a SstI/EcoRI fragment of about 0.3 kb.
BamHI, BglI, BstEII, EcoRI, HindIII and SstI cut outside the VNTR, generating one fragment per allele, but the sizes are difficult to resolve.
A 0.6 kb TaqI fragment has been used to detect the VNTR polymorphism.
TaqI cuts within the VNTR generating 1 to 4 different sized alleles per individual. HinfI also cuts within the VNTR.
References

Heisterkamp N, et al. ABR, an active BCR-related gene. Nucleic Acids Res. 17: 8821-8831, 1989. PubMed: 2587217

DiSilvestre D, et al. A hypervariable RFLP within the ABR gene. Nucleic Acids Res. 18: 5924, 1990. PubMed: 1977147

Deborah Disilvestre, personal communication

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