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當(dāng)前位置: 首頁 > ATCC代理 > pVNTR-B B235673
pVNTR-B
pVNTR-B
規(guī)格:
價格:
編號:B235673
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pVNTR-B
商品貨號
B235673
Designations
pVNTR-B
Species
Homo sapiens, human
培養(yǎng)基
Applications
A 0.6 kb TaqI fragment has been used to detect the VNTR polymorphism.
Vector
Construct size (kb): 6.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 4.199999809265137
Gene product: active BCR-related gene [ABR]
Insert Size (kb)
4.200
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4, 2.6; EcoRI--7.0; HindIII--7.0; BamHI--7.0; PstI--6.6, 0.4.
The insert is the 4.2 kb HindIII/EcoRI fragment containing VTR B and exons 4 and 5. The repeat elements are in the HindIII/XhoI fragment of about 1.8 kb.
The exons are close to the EcoRI end, with exon 5 contained in a SstI/EcoRI fragment of about 0.3 kb.
BamHI, BglI, BstEII, EcoRI, HindIII and SstI cut outside the VNTR, generating one fragment per allele, but the sizes are difficult to resolve.
A 0.6 kb TaqI fragment has been used to detect the VNTR polymorphism.
TaqI cuts within the VNTR generating 1 to 4 different sized alleles per individual. HinfI also cuts within the VNTR.
References

Heisterkamp N, et al. ABR, an active BCR-related gene. Nucleic Acids Res. 17: 8821-8831, 1989. PubMed: 2587217

DiSilvestre D, et al. A hypervariable RFLP within the ABR gene. Nucleic Acids Res. 18: 5924, 1990. PubMed: 1977147

Deborah Disilvestre, personal communication

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