| 產品名稱 |
p3.4 [p3.4BHI] |
| 商品貨號 |
B199076 |
| Designations |
p3.4 [p3.4BHI] |
| Species |
Homo sapiens, human |
| Depositors |
RL White |
| Vector |
Construct size (kb): 7.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IGHJ
Gene name: immunoglobulin heavy polypeptide, joining region
Contains complete coding sequence?: U
Chromosome: 14; Localization: 14 q32.33
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 3.4
Cross references: Insert lengths(kb): 3.400000095367432 Gene product: immunoglobulin heavy polypeptide, joining region [IGHJ] Alleles: A1, A2, A3, A4 |
| Insert Size (kb) |
3.400 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 3.4; BglII--6.4, 1.4; EcoRI--7.8; EcoRV--7.8; PstI--7.8. This fragment originates 1.25 kb 5' to a BglII site just 5' to the J(H) sequences. Subcloned from Ch28-6. Enzymes(s) not detecting polymorphism: SstI, EcoRV, EcoRI, HaeIII, PvuII, PstI, TaqI, NdeII. |
| References |
Silva AJ, et al. Characterization of a highly polymorphic region 5' to JH in the human immunoglobulin heavy chain. Nucleic Acids Res. 15: 3845-3857, 1987. PubMed: 2884636
Raymond L White, personal communication
|
