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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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p3.4 [p3.4BHI]
p3.4 [p3.4BHI]
規格:
價格:
編號:B231029
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p3.4 [p3.4BHI]
商品貨號
B231029
Designations
p3.4 [p3.4BHI]
Species
Homo sapiens, human
培養基
Vector
Construct size (kb): 7.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IGHJ
Gene name: immunoglobulin heavy polypeptide, joining region
Contains complete coding sequence?: U
Chromosome: 14; Localization: 14 q32.33
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 3.4
Cross references:
Insert lengths(kb): 3.400000095367432
Gene product: immunoglobulin heavy polypeptide, joining region [IGHJ]
Alleles: A1, A2, A3, A4
Insert Size (kb)
3.400
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 3.4; BglII--6.4, 1.4; EcoRI--7.8; EcoRV--7.8; PstI--7.8.
This fragment originates 1.25 kb 5' to a BglII site just 5' to the J(H) sequences.
Subcloned from Ch28-6.
Enzymes(s) not detecting polymorphism: SstI, EcoRV, EcoRI, HaeIII, PvuII, PstI, TaqI, NdeII.
References

Silva AJ, et al. Characterization of a highly polymorphic region 5' to JH in the human immunoglobulin heavy chain. Nucleic Acids Res. 15: 3845-3857, 1987. PubMed: 2884636

Raymond L White, personal communication

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