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lambdaPNP2

規格:

價格:

編號:B201244

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

lambdaPNP2

商品貨號

B201244

Designations

lambdaPNP2

Species

Homo sapiens, human

培養基

Vector

Construct size (kb): 44.5

Insert

DNA: genomic

Insert lengths(kb): 14.0

Gene product: nucleoside phosphorylase [NP]

Insert Size (kb)

14.0

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 9.8, 4.4, 3.3, 2.1; HindIII--19.0, 7.0, 5.8, 3.4, 2.1; BamHI--17.0, 6.8, 3.8, 5.4, 3.1, 2.6; PstI--11.0, 6.6, 5.4, 3.3, 2.9, 2.3, 2.0, 1.7, 1.25; SalI--39.0.

This insert contains 5' flanking sequences, exons 1-5, introns 1-4, and part of intron 5. There are 5 EcoRI sites (1 in the 5' flanking sequences, 2 in intron 1, and 1 in intron 2) in the insert. Exon 2 is contained in a 250 bp EcoRI fragment.

There are 3 BamHI (5' flanking, exon 1, intron 1) and 6 HindIII (2 in 5' flanking, 2 in intron 1, 1 in intron 2, 1 in intron 4) sites in the insert.

One intron was missed in the mapping described in the Nucleic Acids Research paper. A partial sequence of a mutant PNP gene has been described.

References

Williams SR, et al. Human purine nucleoside phosphorylase cDNA sequence and genomic clone characterization. Nucleic Acids Res. 12: 5779-5787, 1984. PubMed: 6087295

Williams SR, et al. A human purine nucleoside phosphorylase deficiency caused by a single base change. J. Biol. Chem. 262: 2332-2338, 1987. PubMed: 3029074

B219863：lambdaPNP2

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