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lambdaPNP2
lambdaPNP2
規(guī)格:
價格:
編號:B219863
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
lambdaPNP2
商品貨號
B219863
Designations
lambdaPNP2
Species
Homo sapiens, human
培養(yǎng)基
Depositors
DW Martin
Vector
Construct size (kb): 44.5
Insert
DNA: genomic
Insert lengths(kb): 14.0
Gene product: nucleoside phosphorylase [NP]
Insert Size (kb)
14.0
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 9.8, 4.4, 3.3, 2.1; HindIII--19.0, 7.0, 5.8, 3.4, 2.1; BamHI--17.0, 6.8, 3.8, 5.4, 3.1, 2.6; PstI--11.0, 6.6, 5.4, 3.3, 2.9, 2.3, 2.0, 1.7, 1.25; SalI--39.0.
This insert contains 5' flanking sequences, exons 1-5, introns 1-4, and part of intron 5. There are 5 EcoRI sites (1 in the 5' flanking sequences, 2 in intron 1, and 1 in intron 2) in the insert. Exon 2 is contained in a 250 bp EcoRI fragment.
There are 3 BamHI (5' flanking, exon 1, intron 1) and 6 HindIII (2 in 5' flanking, 2 in intron 1, 1 in intron 2, 1 in intron 4) sites in the insert.
One intron was missed in the mapping described in the Nucleic Acids Research paper. A partial sequence of a mutant PNP gene has been described.
References

Williams SR, et al. Human purine nucleoside phosphorylase cDNA sequence and genomic clone characterization. Nucleic Acids Res. 12: 5779-5787, 1984. PubMed: 6087295

Williams SR, et al. A human purine nucleoside phosphorylase deficiency caused by a single base change. J. Biol. Chem. 262: 2332-2338, 1987. PubMed: 3029074

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