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phIL1AcDNA [human IL-1alpha cDNA]

規格:

價格:

編號:B202598

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

phIL1AcDNA [human IL-1alpha cDNA]

商品貨號

B202598

Designations

phIL1AcDNA [human IL-1alpha cDNA]

GenBank Number

M28983

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 6.400000095367432

Insert

DNA: cDNA

Insert lengths(kb): 2.400000095367432

Tissue: peripheral blood cells (LPS activated)

Gene product: interleukin 1, alpha [IL1]

Insert Size (kb)

2.400

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

The insert contains the following restriction sites (nt positions according to the published sequence): EcoRI--434; HindIII--227; ScaI--1670.

Restriction digests of the clone give the following sizes (kb): BamHI--4.0, 2.4; EcoRI--4.2, 2.2; HindIII--4.1, 2.3; SacI-- uncut; PstI--6.4.

Contains the complete coding sequence (nt 38-852).

pMG-5 is a pBR322 derivative in which the sequence adjacent to the EcoRV site has been modified (GGATATCG to GGATATCC) to allow generation of flanking BamHI sites after homopolymeric tailing EcoRV-cut vector with C's.

References

Gubler U, et al. Recombinant human interleukin 1 alpha: purification and biological characterization. J. Immunol. 136: 2492-2497, 1986. PubMed: 3485152

Ueli Gubler, personal communication

B240500：phIL1AcDNA [human IL-1alpha cDNA]

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