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phIL1AcDNA [human IL-1alpha cDNA]

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價格:

編號:B240500

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

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產品名稱

phIL1AcDNA [human IL-1alpha cDNA]

商品貨號

B240500

Designations

phIL1AcDNA [human IL-1alpha cDNA]

GenBank Number

M28983

培養基

Species

Homo sapiens, human

Depositors

U Gubler

Vector

Construct size (kb): 6.400000095367432

Insert

DNA: cDNA

Insert lengths(kb): 2.400000095367432

Tissue: peripheral blood cells (LPS activated)

Gene product: interleukin 1, alpha [IL1]

Insert Size (kb)

2.400

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

The insert contains the following restriction sites (nt positions according to the published sequence): EcoRI--434; HindIII--227; ScaI--1670.

Restriction digests of the clone give the following sizes (kb): BamHI--4.0, 2.4; EcoRI--4.2, 2.2; HindIII--4.1, 2.3; SacI-- uncut; PstI--6.4.

Contains the complete coding sequence (nt 38-852).

pMG-5 is a pBR322 derivative in which the sequence adjacent to the EcoRV site has been modified (GGATATCG to GGATATCC) to allow generation of flanking BamHI sites after homopolymeric tailing EcoRV-cut vector with C's.

References

Gubler U, et al. Recombinant human interleukin 1 alpha: purification and biological characterization. J. Immunol. 136: 2492-2497, 1986. PubMed: 3485152

Ueli Gubler, personal communication

B202598：phIL1AcDNA [human IL-1alpha cDNA]

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