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Rubella virus
Rubella virus
規(guī)格:
價(jià)格:
編號(hào):B206835
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
Rubella virus
商品貨號(hào)
B206835
Classification
Togaviridae, Rubivirus
Agent
Rubella virus
培養(yǎng)基
Strain
M33
Common Name
Rubella virus
Applications
Respiratory research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Throat washings from Army recruit, New Jersey, 1961
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Rubella virus is 40-80 nm in diameter and ether sensitive.

Vials contain total fluid and material from cell culture infected by virus. The vials will contain cellular debris and possibly cells containing cell-associated virus.
Effect on Host
No CPE produced on production host. Recognized primarily by interference for other viruses (GMK), or production of CF and HA antigens.
Recommended Host
BS-C-1 (ATCC® CCL-26™)
Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 60-70% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 11-15 days
Name of Depositor
PD Parkman, Walter Reed Army Institute of Research
Source
Throat washings from Army recruit, New Jersey, 1961
Year of Origin
1961
References

Parkman PD, et al. Recovery of rubella virus from army recruits. Proc. Soc. Exp. Biol. Med. 111: 225-230, 1962. PubMed: 13941530

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