Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Incubation: 12-14 days at 30°C, a 5% CO2 in air atmosphere is recommended.
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).
Plotkin SA, et al. Studies of immunization with living rubella virus. Trials in children with a strain cultured from an aborted fetus. Am. J. Dis. Child. 110: 381-389, 1965. PubMed: 5834207
Plotkin SA, et al. Attenuation of RA 27/3 rubella virus in WI-38 human diploid cells. Am. J. Dis. Child. 118: 178-185, 1969. PubMed: 5794813
