Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% -90% confluent (not 100% confluent).
Incubation: 1 to 5 days at 37°C, a 5% CO2 in air atmosphere is recommended.
Binn LN, et al. Recovery and characterization of a coronavirus from military dogs with diarrhea. Proc. 78th Ann. Meeting U.S. Anim. Health Assoc. 78: 359-366, 1974. PubMed: 4377955
