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Canine adenovirus 2
Canine adenovirus 2
規格:
貨期:
編號:B225404
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Canine adenovirus 2
商品貨號 B225404
Classification Adenoviridae, Mastadenovirus, Canine adenovirus A
Deposited As Canine adenovirus
Agent Canine adenovirus 2
Strain Toronto A 26/61
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Throat swab from a dog with laryngotracheitis
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-800 Images
Effect on Host CPE, cell rounding and sloughing
Recommended Host MDCK (ATCC CCL-34)
Growth Conditions Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.

Incubation: 3-8 days
Name of Depositor LN Binn, Walter Reed Army Institute of Research, Washington, DC
Source Throat swab from a dog with laryngotracheitis
References

Ditchfield J, et al. Association of a canine adenovirus (Toronto A 26/61) with an outbreak of laryngotracheitis (“Kennel Cough”): A Preliminary Report. Can. Vet. J. 3: 238-247.1962, PubMed: 17421510

Kapsenberg JG. Relationship of infectious canine hepatitis virus to human adenovirus. Proc. Soc. Exp. Biol. Med. 101: 611-614, 1959. PubMed: 14404448

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