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當前位置: 首頁 > ATCC代理 > C303/L2-T1 [C303/L2/T1, C303/L2-T1] B209533
C303/L2-T1 [C303/L2/T1, C303/L2-T1]
C303/L2-T1 [C303/L2/T1, C303/L2-T1]
規格:
價格:
編號:B209533
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
C303/L2-T1 [C303/L2/T1, C303/L2-T1]
商品貨號
B209533
Designations
C303/L2-T1 [C303/L2/T1, C303/L2-T1]
GenBank Number

J02691

培養基
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors
A Tzagoloff
Vector
Construct size (kb): 18.70000076293945
Insert
DNA: genomic
Insert lengths(kb): 8.0
Gene product: phenylalanyl-tRNA synthetase, alpha subunit
Insert Size (kb)
8.0
Genotype
MATalpha leu2-3 leu2-112 msf1-1
Mating Type
alpha
Media
ATCC® Medium 1688: Glycerol enriched medium
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains the following sites separated by (kb) (approximate): SphI- 1.4- SacI- 0.6- HindIII- 1.6- KpnI- HindIII- 0.8- SphI- 1.2- SphI.
The smallest subclone capable of complementing msf1-1, msf1-3, and msf1-4 mutations contained a 2.2 kb SacI/KpnI fragment.
MSF1 is a nuclear gene encoding the alpha subunit of the mitochondrial phenylalanyl-tRNA synthetase.
Carries plasmid pG120/T1 with wild-type gene
The probe, pG120/T1, has been deposited in Escherichia coli RR1 as ATCC 37664 and in Saccharomyces cerevisiae C303/L2 as ATCC 66317.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Koerner TJ, et al. Isolation and characterization of the yeast gene coding for the alpha subunit of mitochondrial phenylalanyl-tRNA synthetase. J. Biol. Chem. 262: 3690-3696, 1987. PubMed: 3029120

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