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當(dāng)前位置: 首頁 > ATCC代理 > C303/L2-T1 [C303/L2/T1, C303/L2-T1] B215950
C303/L2-T1 [C303/L2/T1, C303/L2-T1]
C303/L2-T1 [C303/L2/T1, C303/L2-T1]
規(guī)格:
價格:
編號:B215950
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
C303/L2-T1 [C303/L2/T1, C303/L2-T1]
商品貨號
B215950
Designations
C303/L2-T1 [C303/L2/T1, C303/L2-T1]
GenBank Number

J02691

培養(yǎng)基
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors
A Tzagoloff
Vector
Construct size (kb): 18.70000076293945
Insert
DNA: genomic
Insert lengths(kb): 8.0
Gene product: phenylalanyl-tRNA synthetase, alpha subunit
Insert Size (kb)
8.0
Genotype
MATalpha leu2-3 leu2-112 msf1-1
Mating Type
alpha
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains the following sites separated by (kb) (approximate): SphI- 1.4- SacI- 0.6- HindIII- 1.6- KpnI- 0.6- HindIII- 0.8- SphI- 1.2- SphI.
Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--3.75, 3.5, 3.3, 2.9, 2.3, 2.0, 0.94; XhoI--18.7; XbaI--14.6, 4.2; XbaI/AvaI--4.4, 3.5, 3.1, 2.3, 2.0, 1.8, 1.5; BamHI--18.7.
The smallest subclone capable of complementing msf1-1, msf1-3, and msf1-4 mutations contained a 2.2 kb SacI/KpnI fragment.
MSF1 is a nuclear gene encoding the alpha subunit of the mitochondrial phenylalanyl-tRNA synthetase.
The probe, pG120/T1, has been deposited in Escherichia coli RR1 as ATCC 37664 and in Saccharomyces cerevisiae C303/L2 as ATCC 66317.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Koerner TJ, et al. Isolation and characterization of the yeast gene coding for the alpha subunit of mitochondrial phenylalanyl-tRNA synthetase. J. Biol. Chem. 262: 3690-3696, 1987. PubMed: 3029120

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