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delta p53
delta p53
規格:
價格:
編號:B210472
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
delta p53
商品貨號
B210472
Designations
delta p53
GenBank Number

X02469

培養基
Species
Homo sapiens, human
Depositors
J Kim, R McLinden
Applications
Expression from this construct has been used to quantitate p53 expression levels.
The following primers can be used for RT-PCR to detect transcription of the cloned insert: Forward 5' - CATCTACAAGCAGTCACA - 3'; Reverse 5' - GCAGTGCTCGCTTAGTGC - 3'. The amplified product size is 442 bp.
The following oligonucleotide sequence can be used as an internal probe to detect the amplified product: 5' - ATCCACTACAACTACATG - 3'.
Vector
Construct size (kb): 3.299999952316284
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4Z
Intact vector size: 3.000
Type of vector: plasmid
Vector end: HincII
Vector end: HincII
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TP53
Genomic copy number: unique
Gene name: tumor protein p53 (Li-Fraumeni syndrome)
Contains complete coding sequence?: N
Chromosome: 17; Localization: 17 p13.1
Tissue: SV40-transformed fibroblast
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.6
Cross references: DNA Seq. Acc.: X02469
Nucleotides 1-567 of the insert correspond to
nucleotides 600-1167 of X02469.
Insert lengths(kb): 0.6000000238418579
Tissue: SV40-transformed fibroblast
Gene product: tumor protein p53 (Li-Fraumeni syndrome)( tumor protein p53 (Li-Fraumeni syndrome), p53 cellular tumor antigen) [TP53]
Alleles: D2, C2, C1, A1, A1, B2, B1, D1, A2, A2
Insert Size (kb)
0.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--2.7, 0.6; EcoRI--3.3; BamHI--3.3.
Expression from this construct has been used to quantitate p53 expression levels.
The following primers can be used for RT-PCR to detect transcription of the cloned insert: Forward 5' - CATCTACAAGCAGTCACA - 3'; Reverse 5' - GCAGTGCTCGCTTAGTGC - 3'. The amplified product size is 442 bp.
The following oligonucleotide sequence can be used as an internal probe to detect the amplified product: 5' - ATCCACTACAACTACATG - 3'.
Insert was isolated from php53B plasmid DNA (ATCC 57255) and subcloned into a vector allowing RNA transcription.
References

Zakut-Houri R, et al. Human P53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4: 1251-1255, 1985. PubMed: 4006916

Robert McLinden, personal communication

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