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pLGdelta312

規(guī)格:

價(jià)格:詢價(jià)

編號(hào):B242304

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pLGdelta312

商品貨號(hào)

B242304

Designations

pLGdelta312

Depositors

DJ Stillman

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 10.1999998092651400

Vector: pLGdelta312 (plasmid)

Promoters: Promoter CYC1 promoter (including the CYC1 UAS)

Construction: pBR322

Marker(s):URA3,ampR

Construct size (kb): 10.19999980926514

Features: marker(s): URA3
marker(s): ampR
promoter: CYC1 promoter (including the CYC1 UAS)
replicon: 2 micron ori
replicon: pMB1
coding sequence: lacZ

Applications

YE-type (episomal) shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): SmaI--10.3; HindIII--8.0, 2.1; EcoRI--4.5, 3.15, 2.25.

This vector is one of three lacZ reporter plasmids that contain UAS (upstream activation site) elements (ATCC 87511, 87512 & 87522). They differ by polylinker sites.

DNA fragments can be inserted into these plasmids to screen for DNA elements that reduce promoter activity.

The vector was constructed by Lenny Guarente.

Growth Conditions

Temperature: 37.0°C

References

Guarente L, Hoar E. Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the "TATA" box. Proc. Natl. Acad. Sci. USA 81: 7860-7864, 1984. PubMed: 6096863

Related Products

component of:ATCC 87540

Shipped

freeze-dried

B207969：pLGdelta312-polylinker

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