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當(dāng)前位置: 首頁(yè) > ATCC代理 > Vaccinia virus B210515
Vaccinia virus
Vaccinia virus
規(guī)格:
價(jià)格:
編號(hào):B210515
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Vaccinia virus
商品貨號(hào)
B210515
Classification
Poxviridae, Orthopoxvirus
Agent
Vaccinia virus
培養(yǎng)基
Strain
CL
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
?-70°C or colder
Comments
VR-1774 is produced in BSC-40 cells, which is a change in cell line from ATCC VR-117 to increase titer.
Effect on Host
?CPE, refractile cell rounding
CPE, refractile cell rounding
Recommended Host

Production Host: ?BSC-40 (ATCC? CRL-2761™)

BSC-40 (ATCC CRL-2761)
Growth Conditions
Temperature: 37°C
Growth Conditions: Plate cells 24-48 hours in advance and infect when cultures are 90 to 95% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide an optimal MOI (e.g. 0.1). Adsorb 30 minutes at 37°C in a humidified 5% CO2 atmosphere, rocking every 7-10 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-3 days
Name of Depositor
F Fenner, Australian National University
Source
Most likely derived from New York City Department of Health strain of vaccinia virus. Cell culture adapted to BSC-40 cells.
References

Fenner, F and Burnet, FM. A short description of the Poxvirus group (Vaccinia and related viruses). Virology 4: 305-314, 1957. PubMed: 13496547.

Craigie J. . Br. J. Exp. Pathol. 13: 259, 1932.

Parker RF, et al. Further studies of the infectious unit of Vaccinia. J. Exp. Med. 74: 263-281, 1941.

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