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pLUC Phagemid in Escherichia coli HB101

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編號:B212418

品牌:Mingzhoubio

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產品名稱

pLUC Phagemid in Escherichia coli HB101

商品貨號

B212418

Designations

pLUC Phagemid in Escherichia coli HB101

Depositors

A Ward

培養基

Biosafety Level

Vector Information

Size (kb): 5.40

Vector: pLUC (phagemid)

Promoters: Promoter lac

Construction: pSVOALdelta5', pBluescriptSK+

Marker(s):ampR

Construct size (kb): 5.40

Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1, f1
terminator: SV40 polyadenylation

Applications

promoter-cloning vector

vector containing primer sites useful for sequencing

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--5.4; HindIII--5.4; XhoI--5.4.

The AUG start codon is 22bp downstream from the HindIII site within the luc gene.

Differs from pLUCS (ATCC 77415) only in the polylinker.

Permits beta-galactosidase blue/white color selection for identification of recombinants. The color is weaker than from the parent vector and transformed cells may need to be grown 18-24 hours at 37C.

Promoter-cloning vector for analysis of eukaryotic promoters, using the luciferase reporter gene.

Constructed from pSVOALdelta5' by inserting a 2.6 kb BamHI fragment, containing the firefly (Photinus pyralis) luciferase gene and SV40 sequences into the BamHI site of pBluescriptSK+, followed by the removal of the 3' BamHI site.

The order of the major features in the phagemid is: 3' lacZ - XhoI/MCS/BamHI - 5' luc 3' - SV40 small t intron - SV40 polyadenylation signal - NotI - SstII - 5' lacZ - pMB1 ori - ampR - f1 ori.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727

Caricasole A, Ward A. A luciferase-reporter vector with blue-white selection for rapid subcloning and mutational analysis of eukaryotic promoters. Gene 124: 139-140, 1993. PubMed: 8382654

Caricasole A, Ward A. Transactivation of mouse insulin-like growth factor II (TGF-II) gene promoters by the AP-1 complex. Nucleic Acids Res. 21: 1873-1879, 1993. PubMed: 8493103

Shipped

freeze-dried

Shipping Information

Freeze dried phagemid in E. coli

B219781：pLUC+

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