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EBO-pCD-Leu2
EBO-pCD-Leu2
規格:
價格:
編號:B213051
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
EBO-pCD-Leu2
商品貨號
B213051
Designations
EBO-pCD-Leu2
Species
Homo sapiens, human
培養基
Depositors
RF Margolskee
Applications
DNA from secondary transfectants was used to isolate the clone.
Vector
Construct size (kb): 12.89999961853027
Insert
DNA: cDNA
Insert lengths(kb): 2.299999952316284
Tissue: JM cell line DNA transferred into J107 cells
Gene product: antigen CD8A (p32)( CD8 antigen, alpha-chain, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, T8 T-cell antigen) [CD8]
Insert Size (kb)
2.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Digestion with BamHI yields 2 vector fragments (5.4, 5.2 kb) and the 2.3 kb insert (+ some pcD).
Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.0; SfiI--7.6, 5.4; PstI--7.4, 3.9, 1.5; SacI--13.0; BamHI--5.2 doublet, 2.3; HindIII--13.0; BglII--13.0.
Vector contains EBV oriP and EBNA-1 to ensure replication in absence of EBNA. Maintained as episomes as 2-10 copies per lymphoblastoid cell; stable as long as selection is applied.
J107 cells were transformed with DNA from the T-lymphoma JM cell line. DNA from secondary transfectants was used to isolate the clone.
References

Margolskee RF, et al. Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol. Cell. Biol. 8: 2837-2847, 1988. PubMed: 2841588

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