国产精品综合视频,一区免费在线观看,2020国产精品

Endothelial Cell Growth Kit-BBE

規格:

價格:

編號:B214283

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

Endothelial Cell Growth Kit-BBE

商品貨號

B214283

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen 1 kit

培養基

Storage Conditions

-20°C (or -70°C for long-term storage)

Comments

The Endothelial Cell Growth Kit-BBE contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete cell culture environment for endothelial cells isolated from large vessels. The final concentration for each component in complete endothelial growth medium is as follows:

Bovine brain extract (BBE): 0.2%
rh EGF: 5 ng/mL
L-glutamine: 10 mM
Heparin sulfate: 0.75 Units/mL
Hydrocortisone: 1 µg/mL
Ascorbic acid: 50 µg/mL
Fetal bovine serum: 2%

Complete Growth Medium

Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.

Table 1. If using the Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), add the indicated volume for each component:

Component

Volume

Sterility Tests

Final Concentration

Functional Tests

Bovine Brain Extract (BBE)

C of A

1.0 mL

B236167：Primary Umbilical Vein Endothelial Cells; Normal, Human, Pooled (HUVEC)

B208382：Microvascular Endothelial Cell Growth Kit-VEGF

B202603：Microvascular Endothelial Cell Growth Kit-BBE

B198288：Primary Pulmonary Artery Endothelial Cells; Normal, Human (HPAEC)

B197777：Primary Umbilical Vein Endothelial Cells; Normal, Human (HUVEC)

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479