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p426 MET25
p426 MET25
規格:
貨期:
編號:B215104
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p426 MET25
商品貨號
B215104
Designations
p426 MET25
Depositors
M Funk
Biosafety Level
1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.3379998207092290
DESCRIPTION OF VECTOR:
Intact vector size: 6.338
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS426 (ATCC 77107), MET25 promoter
Host range: Candida robustaSaccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1300
terminator: CYC1, ->, 2004-2264
MCS: XhoI...SpeI, ->, 2265-2322
promoter for expression: MET25, <-, 2339-2720
marker(s): ampR, <-, 3838-4768
replicon: 2 micron, ->, 4868-6208
Vector: p426 MET25 (plasmid)
Promoters: Promoter for expression MET25
Construction: pRS426 (ATCC 77107), MET25 promoter
Marker(s):URA3,ampR
Construct size (kb): 6.337999820709229
Features: marker(s): URA3
marker(s): ampR
promoter for expression: MET25
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.4; EcoRV--4.7, 1.7; PstI--4.5, 1.9.
Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter is repressed when cells are grown in the presence of methionine.
High copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

Related Products
component of:ATCC 87670
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freeze-dried
Shipping Information
Distributed: freeze-dried
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