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Canid herpesvirus 1

規格:

價格:

編號:B215360

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Canid herpesvirus 1

商品貨號

B215360

Classification

Herpesviridae, Varicellovirus

Agent

Canid herpesvirus 1

培養基

Strain

F 205 (Carmichael)

Common Name

Canine herpesvirus

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen

Storage Conditions

-70°C

Comments

Derived by adaptation of primary cell culture product ATCC VR-647 to MDCK cells (ATCC CCL-34). ATCC VR-647 and ATCC VR-1785 have not been compared for sequence or infectivity in primary cell culture and tissue culture. Chloroform/ether sensitive, not known to hemagglutinate, sensitive to pH below 6.5 and heat labile.

Effect on Host

CPE, cell rounding and sloughing

Recommended Host

MDCK (ATCC CCL-34)

Growth Conditions

Temperature: 37°

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted to provide an optimal MOI 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation: 1-4 days

Name of Depositor

JH Gillespie, New York State Veterinary College, Cornell University

Source

Kidney from 2-3 weeks old puppy

References

Carmichael LE, et al. Clinical and pathologic features of a fatal viral disease of newborn pups. Am. J. Vet. Res. 26: 803-814, 1965. PubMed: 5892835

Carmichael LE, et al. Identification of a cytopathogenic agent infectious for puppies as a Canine herpesvirus. Proc. Soc. Exp. Biol. Med. 120: 644-650, 1965. PubMed: 4221827

B241204：Canid herpesvirus 1

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