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Canid herpesvirus 1

規格:

價格:

編號:B241204

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Canid herpesvirus 1

商品貨號

B241204

Classification

Herpesviridae, Varicellovirus

Deposited As

Canine herpesvirus

培養基

Agent

Canid herpesvirus 1

Strain

D 004

Common Name

Canine herpesvirus

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation

Respiratory tract of a dog

Product Format

frozen 1.0 mL

Storage Conditions

-70°C or colder

Images

Comments

No cross-neutralization with other herpesviruses. Can only be propagated in cells of canine origin. Purified 1 time by terminal dilution, and 3 times by plaque passage.

Effect on Host

CPE, cell rounding and sloughing

Recommended Host

MDCK cells (ATCC^® CCL-34™)

Growth Conditions

Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm²). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-6 days at 37°C in a humidified 5% CO₂ atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor

LN Binn, Division of Veterinary Medicine, Walter Reed Army Institute of Research

Source

Respiratory tract of a dog

References

Binn LN, et al. Viruses recovered from laboratory dogs with respiratory disease. Proc. Soc. Exp. Biol. Med. 126: 140-145, 1967. PubMed: 4294430

Cross References

Nucleotide (GenBank) : U63459 Canine herpesvirus DNA polymerase gene, partial cds.

B215360：Canid herpesvirus 1

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