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YIp5 [pRB12]

規格:

價格:

編號:B217126

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

YIp5 [pRB12]

商品貨號

B217126

Designations

YIp5 [pRB12]

Depositors

D Botstein

培養基

Biosafety Level

Vector Information

Size (kb): 5.5409998893737790

Vector: YIp5 (plasmid)

Construction: pBR322, lambda590-Sc2904

Marker(s):URA3,ampR,tetR

Construct size (kb): 5.540999889373779

Features: marker(s): ampR, tetR, URA3
replicon: pMB1
terminator: none
enhancer: none

Applications

YI-type (integrating) shuttle vector

integrating vector

shuttle vector

Comments

YI-type integrating shuttle vector.

This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 from lambda590-Sc2904 into the AvaI site of pBR322. The 5' - 3' orientation of URA3 is clockwise; SmaI and AvaI sites in the 3' of URA3 are close to the PvuII site of pBR322.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863