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YIp5 [pRB12]

規格:

貨期:

編號:B217126

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱	YIp5 [pRB12]
商品貨號	B217126
Designations	YIp5 [pRB12]
Depositors	D Botstein
Biosafety Level	1
Vector Information	Size (kb): 5.5409998893737790 Vector: YIp5 (plasmid) Construction: pBR322, lambda590-Sc2904 Marker(s):URA3,ampR,tetR Construct size (kb): 5.540999889373779 Features: marker(s): ampR, tetR, URA3 replicon: pMB1 terminator: none enhancer: none
Applications	YI-type (integrating) shuttle vector integrating vector shuttle vector
Comments	YI-type integrating shuttle vector. This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 from lambda590-Sc2904 into the AvaI site of pBR322. The 5' - 3' orientation of URA3 is clockwise; SmaI and AvaI sites in the 3' of URA3 are close to the PvuII site of pBR322.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030 Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863
Related Products	component of:ATCC 77188
Shipped	frozen
Shipping Information	Distributed: freeze-dried

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