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AA5
AA5
規格:
價格:
編號:B221709
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
AA5
商品貨號
B221709
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The AA5 antibody restores strong adhesion to activin-treated blastomeres.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Animals were immunized with purified CEC1-5 (soluble C-cadherin ectodomain) protein.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against C-cadherin
Cellular Products
immunoglobulin; monoclonal antibody; against C-cadherin
Comments
Animals were immunized with purified CEC1-5 (soluble C-cadherin ectodomain) protein.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
C-cadherin is the major cadherin expressed in the blastula and early gastrula and is largely responsible for holding cells in the embryo together.
The AA5 antibody is the first antibody that can activate cadherin adhesive function.
The AA5 antibody restores strong adhesion to activin-treated blastomeres. The C-cadherin-activating antibody strongly inhibits the elongation of animal caps, in response to activin, without affecting mesodermal gene expression.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 5 X 10(4) and 8 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 45% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
mouse kappa
Name of Depositor
BM Gumbiner
Deposited As
mouse (B cell); mouse (myeloma)
References

Zhong Y, et al. Analysis of C-cadherin regulation during tissue morphogenesis with an activating antibody. J. Cell Biol. 144: 351-359, 1999. PubMed: 9922460

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