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p640
p640
規(guī)格:
價(jià)格:
編號(hào):B224844
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
p640
商品貨號(hào)
B224844
Designations
p640
Species
Homo sapiens, human
培養(yǎng)基
Depositors
RA Weinberg
Applications
NIH 3T3 cells were transformed with DNA from the colon adenocarcinoma SW480 cell line to produce the cell line, SW2-3. DNA from SW2-3 was used to isolate the clone.
Vector
Construct size (kb): 4.940000057220459
Insert
DNA: genomic
Insert lengths(kb): 0.6399999856948853
Tissue: SW480 cell line DNA transferred into NIH 3T3 cells
Gene product: Kirsten rat sarcoma 2 viral (v-Ki-ras2) oncogene homolog( c-Kirsten-ras proto-oncogene, oncogene KRAS2, cellular c-Ki-ras2 proto-oncogene) [KRAS2]
Insert Size (kb)
0.640
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; PstI--5.0; EcoRI/HindIII--4.4, 0.65.
Encodes an intron subcloned from DNA of a secondary transformant cloned into the EcoRI site of Charon 16A.
This probe contains a unique sequence derived from a 2.6 kb EcoRI fragment that is present in all transfectants containing the SW480 oncogene.
NIH 3T3 cells were transformed with DNA from the colon adenocarcinoma SW480 cell line to produce the cell line, SW2-3. DNA from SW2-3 was used to isolate the clone.
References

McCoy MS, et al. Characterization of a human colon/lung carcinoma oncogene. Nature 302: 79-81, 1983. PubMed: 6298638

Janssen JW, et al. Ras gene mutations in acute and chronic myelocytic leukemias, chronic myeloproliferative disorders, and myelodysplastic syndromes. Proc. Natl. Acad. Sci. USA 84: 9228-9232, 1987. PubMed: 3122217

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