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p4
p4
規格:
價格:
編號:B226616
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p4
商品貨號
B226616
Designations
p4
GenBank Number

L10918

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 5.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: XhoI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CMKBR1
Gene name: chemokine (C-C) receptor 1
Contains complete coding sequence?: Y
Chromosome: 3; Localization: 3 p21
Tissue: HL-60 cell line
Disease: promyelocytic leukemia
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI linker
Insert 3' end: Modification: XhoI linker
Insert size (kb): 2.156
Cross references: DNA Seq. Acc.: L10918
Nucleotides 1-2156 of the insert correspond to
nucleotides 1-2156 of L10918.
Insert lengths(kb): 2.155999898910523
Tissue: HL-60 cell line promyelocytic leukemia
Disease: promyelocytic leukemia
Gene product: chemokine (C-C) receptor 1( chemokine (C-C) receptor 1, RANTES receptor) [CMKBR1]
Insert Size (kb)
2.156
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--3.0, 2.2; EcoRI--4.6, 0.6; XhoI--5.2; EcoRI/XhoI--3.0, 1.6, 0.6; PstI--4.6, 0.6.
The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.45; EcoRI--0.59; PstI--0.61.
References

Gao JL, et al. Structure and functional expression of the human macrophage inflammatory protein 1alpha/RANTES receptor. J. Exp. Med. 177: 1421-1427, 1993. PubMed: 7683036

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