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p52 c- [p52C-] plasmid in E. coli
p52 c- [p52C-] plasmid in E. coli
規(guī)格:
價(jià)格:
編號(hào):B227853
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
p52 c- [p52C-] plasmid in E. coli
商品貨號(hào)
B227853
Designations
p52 c- [p52C-] plasmid in E. coli
Species
Homo sapiens, human
培養(yǎng)基
Depositors
RA Weinberg
Applications
Contains the 3' untranslated region and has been used to detect transcripts.
Vector
Construct size (kb): 4.2
Insert
DNA: genomic
Insert lengths(kb): 1.5
Tissue: HL-60 cell line (promyelocytic leukemia)
Gene product: neuroblastoma RAS viral (v-ras) oncogene homolog( v-ras neuroblastoma RAS viral oncogene homolog, proto-oncogene N-ras) [NRAS]
Alleles: A1, A2
Insert Size (kb)
1.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.85, 1.3; EcoRI--2.7, 1.45.
Contains the 3' untranslated region and has been used to detect transcripts. The insert contains AccI and PstI sites.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
EcoRI linkers were added during construction and cloned into EcoRI site of pUC12.
References

Murray MJ, et al. The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors. Cell 33: 749-757, 1983. PubMed: 6683594

Barbara Weiffenbach, personal communication

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