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Escherichia coli (Migula) Castellani and Chalmers
Escherichia coli (Migula) Castellani and Chalmers
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B228497
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Escherichia coli (Migula) Castellani and Chalmers
商品貨號(hào)
B228497
Classification
Enterobacteriaceae, Escherichia
Strain Designations
HB101 F'lac::Tn1739tnpR
培養(yǎng)基
Application
transformation host
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen
Shipping Information

Shipped: ?Freeze dried Escherichia coli

Storage Conditions
Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol®
no
Genotype
F' lac::Tn1739tnpR lacY1 galK2 leuB6 proA2 thi recA13 rpsL20 delta(mcrC-mrr) xyl-5 mtl-1 supE44
Type Strain
no
Comments
A chloramphenicol resistant host, expressing tnpR resolvase and the cI lambda repressor, for use in the Tn1721-derived site-specific recombination systems and excision of plasmids from the vector lambdaRESII (ATCC 77404).
Phenotype verified: gal, lac, strR, leu, pro, thi, supE, F', r-m-, cmlR.
Contains two copies of the tnpR gene, one under control of the tac promoter for increased inducible expression.
The order of the major features in the transposon is: inverted repeats - EcoRI - tnpR - tac promoter - EcoRI - BamHI - cI - PstI - cmlR - HindIII - res resolution site - tnpR - tnpA - EcoRI - inverted repeats.
Medium
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions

Temperature: 37.0°C

Atmosphere: aerobic

Name of Depositor
J Altenbuchner
References

Altenbuchner J. A new lambda RES vector with a built-in Tn1721-encoded excision system. Gene 123: 63-68, 1993. PubMed: 8380784

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