Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus diluted with VGM to provide a MOI of about 0.1. Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding VGM.
Incubation: Incubate infected Vero cell culture for 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance through 90% of the culture. Incubate for 3 days on CAM.
