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6.12
6.12
規(guī)格:
價(jià)格:
編號(hào):B236915
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
6.12
商品貨號(hào)
B236915
Organism
Mus musculus, mouse
Tissue
spleen
培養(yǎng)基
Cell Type
hybridoma: lymphoblast B lymphocyte; somatic cell hybrid
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, with some loosely adherent cells
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody specifically binds to an extrcellular domain of human vascular endothelial growth factor (VEGF) receptor 1 [fms-like tyrosine kinase (Flt-1) [U.S. Pat.
Storage Conditions
liquid nitrogen vapor phase
Comments
The antibody specifically binds to an extrcellular domain of human vascular endothelial growth factor (VEGF) receptor 1 [fms-like tyrosine kinase (Flt-1) [U.S. Pat. 6,365,157; U.S. PAT. 6,448,077]. The 6.12 cell line was deposited at the ATCC as PTA-3344. Progeny from PTA-3344 are available as CRL-13006.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 x 104 viable cells/mL. Adherent cells may be removed by shaking the flask. Maintain cell density between 3 x 104 and 4 x 105 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgG1 kappa
Name of Depositor
ImClone Systems Inc.
References

Rockwell P, Goldstein NI. Monoclonal antibodies specific to VEGF receptors and uses thereof. US Patent 6,365,157 dated Apr 2 2002

Rockwell P, Goldstein NI. Chimeric and humanized monoclonal antibodies specific to VEGF receptors. US Patent 6,448,077 dated Sep 10 2002

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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