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YCpIF1

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價格:

編號:B241346

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

YCpIF1

商品貨號

B241346

Designations

YCpIF1

Depositors

RW Davis, PK Foreman

培養基

Biosafety Level

Vector Information

Size (kb): 7.5000000000000000

Vector: YCpIF1 (plasmid)

Promoters: Promoter for expression GAL1

Construction: pUN105

Marker(s):LEU2,ampR

Construct size (kb): 7.5

Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GAL1
replicon: ARS1
replicon: pMB1
MCS: SacI...SalI
centromere: CEN4

Applications

YX-type (expression) shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): XbaI--7.5; BamHI--7.5; EcoRI--4.3, 3.5.

One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter.

Vectors YCpIF1, YCpIF2 and YCpIF3 (ATCC 87078-87080) differ only in the reading frame of the multiple cloning site.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907