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Canine distemper virus
Canine distemper virus
規(guī)格:
貨期:
編號:B163006
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Canine distemper virus
商品貨號
B163006
Classification
Paramyxoviridae, Morbillivirus
Deposited As
Canine distemper
Agent
Canine distemper virus
Strain
Lederle
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Images
ATCC VR-128 Canine distemper virus
Comments
Belongs to the same virus subgroup as measles and rinderpest.
Effect on Host
CPE, refractile cell rounding and degeneration
Recommended Host
SL-29 (ATCC® CRL-1590™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-8 days. Replace virus growth medium every 2-3 days.
Name of Depositor
VJ Cabasso, PJ Vasington, Lederle Laboratories Division of American Cyanamid Company
Source
Field case of distemper encephalitis in dog
References

Cabasso VJ, Cox HR. A distemper-like strain of virus derived from a case of canine encephalitis: its adaptation to the chick embryo and subsequent modification. Cornell Vet. 42: 96-107, 1952. PubMed: 14905890

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