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Canine parvovirus
Canine parvovirus
規格:
貨期:
編號:B163251
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Canine parvovirus
商品貨號
B163251
Classification
Parvoviridae, Parvovirus, Feline panleukopenia virus
Agent
Canine parvovirus
Strain
C-780916
Applications
Antigenically indistinguishable from feline panleukopenia virus and mink enteritis virus, but it has a broader host range and different HA spectrum, especially as regards pH conditions.
Hemagglutinates swine, Rh+ monkey & cat RBC at pH 6.0-7.5. On passage, add virus 2 hrs after setting host cells.
Antigenically indistinguishable from feline panleukopenia virus and mink enteritis virus, but it has a broader host range and different HA spectrum, especially as regards pH conditions.
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Feces of dog with hemorrhagic enteritis
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Non-pathogenic for neonatal pups. Antigenically indistinguishable from Feline panleukopenia virus and Mink enteritis virus, but has broader host range and different HA spectrum, especially in regards to pH conditions. Hemagglutinates porcine, Rh+ monkey, and feline red blood cells at pH 6.0-7.5. May fail to immunize dogs if passaged 8-10 times in feline cells.
Effect on Host
CPE, refractile cells, cell rounding and sloughing
Recommended Host
A-72 (ATCC® CRL-1542™)
Growth Conditions
Recommendations for Infection: Plate cells 2-4 hours prior to infection and infect when cultures are 30-40% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 3-9 days at 35°C in a humidified atmosphere, until CPE are well advance throughout.
Virus Growth Medium: Leibovitz's L-15 Medium (ATCC 30-2008) + 2% FBS (ATCC 30-2020)
Name of Depositor
LE Carmichael, New York State College of Veterinary Medicine, Cornell University
Source
Feces of dog with hemorrhagic enteritis, 1978
References

Appel MJ, et al. Isolation and immunisation studies of a canine parco-like virus from dogs with haemorrhagic enteritis. Vet. Rec. 105: 156-159, 1979. PubMed: 516347

Carmichael LE, et al. A modified live canine parvovirus strain with novel plaque characteristics. I. Viral attenuation and dog response. Cornell Vet. 71: 408-427, 1981. PubMed: 7318443

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