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當(dāng)前位置: 首頁 > ATCC代理 > HeLa B164591
HeLa
HeLa
規(guī)格:
價格:
編號:B164591
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HeLa
商品貨號
B164591
Organism
Homo sapiens, human
Tissue
cervix
培養(yǎng)基
Cell Type
epithelial
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2  [Cells contain human papilloma virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
adenocarcinoma
Age
31 years adult
Gender
female
Ethnicity
Black
Applications
These cells are a suitable transfection host.
This cell line can be used to screen for Escherichia coli strains with invasive potential.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
Modal number = 82; range = 70 to 164.
There is a small telocentric chromosome in 98% of the cells. 100% aneuploidy in 1385 cells examined. Four typical HeLa marker chromosomes have been reported in the literature. HeLa Marker Chromosomes: One copy of Ml, one copy of M2, four-five copies of M3, and two copies of M4 as revealed by G-banding patterns. M1 is a rearranged long arm and centromere of chromosome 1 and the long arm of chromosome 3. M2 is a combination of short arm of chromosome 3 and long arm of chromosome 5. M3 is an isochromosome of the short arm of chromosome 5. M4 consists of the long arm of chromosome 11 and an arm of chromosome 19. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Images
Clinical Data
31 years
Black
female
HeLa Markers
Y
Genes Expressed
Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway. The cells are positive for keratin by immunoperoxidase staining.
Virus Susceptibility
Human adenovirus 3
Encephalomyocarditis virus
Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Comments
The cells are positive for keratin by immunoperoxidase staining.
HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences.
P53 expression was reported to be low, and normal levels of pRB (retinoblastoma suppressor) were found.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor
WF Scherer
Deposited As
Homo sapiens
References

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Cross References

Nucleotide (GenBank) : X98561 H.sapiens mRNA for dsRNA adenosine deaminase, exon 1C.

Nucleotide (GenBank) : X98562 H.sapiens mRNA for dsRNA adenosine deaminase, exon 1D.

Nucleotide (GenBank) : U89349 Human papillomavirus type 18 variant, partial sequence.

Nucleotide (GenBank) : AJ251709 Homo sapiens partial mRNA for putative protein kinase ERK3.

Nucleotide (GenBank) : X84958 H.sapiens mRNA for interferon-induced 17kDa membrane protein.

Nucleotide (GenBank) : X98559 H.sapiens mRNA for dsRNA adenosine deaminase, constant region.

Nucleotide (GenBank) : AJ251708 Homo sapiens partial mRNA for putative microtubule-binding protein.

Nucleotide (GenBank) : NM_003641 Homo sapiens interferon induced transmembrane protein 1 (9-27) (IFITM1), mRNA.

Nucleotide (GenBank) : NM_022126 Homo sapiens phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP), mRNA.

Nucleotide (GenBank) : AB049629 Homo sapiens lhpp mRNA for phospholysine phosphohistidine inorganic pyrophosphate phosphatase, complete cds.

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