Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The cells are positive for keratin by immunoperoxidase staining.
A culture at approximately passage 400 was submitted to the American Type Culture Collection in February, 1972.
HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. ATCC confirmed this cell line is positive for the presence of Papillomavirus viral DNA sequences via PCR.
Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. PubMed: 3180844
Boshart M, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740
Puck TT, et al. Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer. J. Exp. Med. 103: 273-283, 1956. PubMed: 13286432
Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
Puck TT, Marcus PI. A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: the use of x-irradiated cells to supply conditioning factors. Proc. Natl. Acad. Sci. USA 41: 432-437, 1955.
Puck TT, Fisher HW. Genetics of Somatic Mammalian Cells. J. Exp. Med. 104: 427-434, 1956. PubMed: 19867118
Ham RG, Puck TT. Quantitative colonial growth of isolated mammalian cells. Methods Enzymol 5 90-119 1962
Darnell JE Jr., et al. The effect of cell population density on the amino acid requirements for poliovirus synthesis in HeLa cells. J. Exp. Med. 110: 445-450, 1959. PubMed: 13814142
Cohen EP, Eagle H. A simplified chemostat for the growth of mammalian cells: characteristics of cell growth in continuous culture. J. Exp. Med. 113: 467-474, 1961.
Darnell JE Jr., Sawyer TK. Variation in plaque-forming ability among parental and clonal strains of HeLa cells. Virology 8: 223-229, 1959. PubMed: 13669339
Sato G, et al. Molecular growth requirements of single mammalian cells. Science 126: 461-464, 1957. PubMed: 13486039
Soares K, et al. cis-Acting elements involved in transcriptional regulation of the herpes simples virus type 1 latency-associated promoter 1 (LAP1) in vitro and in vivo. J. Virol. 70: 5384-5394, 1996. PubMed: 8764049
Chang YE, et al. Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. J. Virol. 70: 3938-3946, 1996. PubMed: 8648731
Jiang BH, et al. Hypoxia-inducible factor 1 levels vary exponentially over a physiologically relevant range of O2 tension. Am. J. Physiol. 271: C1172-C1180, 1996. PubMed: 8897823
Genuario RR, Perry RP. The GA-binding protein can serve as both an activator and repressor of ribosomal protein gene transcription. J. Biol. Chem. 271: 4388-4395, 1996. PubMed: 8626789
Ladner RD, et al. Identification of a consensus cyclin-dependent kinase phosphorylation site unique to the nuclear form of human deoxyuridine triphosphate nucleotidohydrolase. J. Biol. Chem. 271: 7752-7757, 1996. PubMed: 8631817
Ladner RD, et al. Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase. J. Biol. Chem. 271: 7745-7751, 1996. PubMed: 8631816
Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793
Nucleotide (GenBank) : Y09723 H.sapiens mRNA for Miz-1 protein.
Nucleotide (GenBank) : Y08698 H.sapiens mRNA for RanBP3 (59 kDa).
Nucleotide (GenBank) : Y08699 H.sapiens mRNA for RanBP3, splice variant.
Nucleotide (GenBank) : U25182 Human antioxidant enzyme AOE37-2 mRNA, complete cds.
Nucleotide (GenBank) : U33822 Human tax1-binding protein TXBP181 mRNA, complete cds.
Nucleotide (GenBank) : U43430 Human epsilon isoform 14-3-3 protein mRNA, complete cds.
Nucleotide (GenBank) : Y08697 H.sapiens mRNA for RanBP3 small splice variant (53 kDa).
Nucleotide (GenBank) : L15328 Saccharomyces cerevisiae RNA helicase gene, complete cds.
Nucleotide (GenBank) : U33821 Homo sapiens tax1-binding protein TXBP151 mRNA, complete cds.
Nucleotide (GenBank) : NM_003443 Homo sapiens zinc finger protein 151 (pHZ-67) (ZNF151), mRNA.
Nucleotide (GenBank) : AF034102 Homo sapiens NBMPR-insensitive nucleoside transporter ei (ENT2) mRNA, complete cds.
Nucleotide (GenBank) : NM_006024 Homo sapiens Tax1 (human T-cell leukemia virus type I) binding protein 1 (TAX1BP1), mRNA.
