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T4 Clone 5 (10-0101, 0062-83)
T4 Clone 5 (10-0101, 0062-83)
規格:
價格:詢價
編號:B165817
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
T4 Clone 5 (10-0101, 0062-83)
商品貨號
B165817
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against thyroxine (T4, 3,5,3',5'-tetraiodo-L-thyronine)
Cellular Products
immunoglobulin; monoclonal antibody; against thyroxine (T4, 3,5,3',5'-tetraiodo-L-thyronine)
Comments
Animals were immunized with T4 conjugated to chicken gamma globulin.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The extent of cross-reaction with triiodothyronine (T3) is approximately 2%.
Complete Growth Medium
Culture medium: RPMI 1640 with 2 mM L-glutamine adjusted to contain 1.5 g/L
sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 92.5%; DMSO, 7.5%
Isotype
IgG1
Name of Depositor
Becton, Dickinson and Co.
U.S. Patent Number
References

Siebert GR, Armstrong J. Monoclonal antibodies recognizing L-thyroxine. US Patent 4,636,478 dated Jan 13 1987

Siebert GR, Armstrong J. Monoclonal antibodies recognizing L-thyroxine. US Patent 4,888,296 dated Dec 19 1989

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