Vector Information
Size (kb): 5.4159998893737790
DESCRIPTION OF VECTOR:
Intact vector size: 5.416
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS414
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 200-1213
terminator: CYC1, ->, 1895-2155
MCS: SalI...XbaI, ->, 2161-2219
promoter for expression: TEF, <-, 2219-2621
marker(s): ampR, <-, 3868-4798
replicon: CEN6/ARSH4, ->, 4902-5416
Vector: p414 TEF (plasmid)
Promoters: Promoter for expression TEF
Construction: pRS414
Marker(s):TRP1,ampR
Construct size (kb): 5.415999889373779
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: TEF
replicon: CEN6/ARSH4
MCS: SalI...XbaI
terminator: CYC1
