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p414 GALS

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編號:B228224

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

p414 GALS

商品貨號

B228224

Designations

p414 GALS

Depositors

M Funk

培養基

Biosafety Level

Host

Distribution host: Escherichia coli HB101 (ATCC 33694)

Vector Information

Size (kb): 5.4289999008178710

DESCRIPTION OF VECTOR:
Intact vector size: 5.429
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS414, GALS promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 200-1213
terminator: CYC1, ->, 1895-2155
MCS: XhoI...SpeI, ->, 2155-2212
promoter for expression: GALS, <-, 2219-2634
marker(s): ampR, <-, 3881-4811
centromere: CEN6/ARSH4, ->, 4915-5429

Vector: p414 GALS (plasmid)

Promoters: Promoter for expression GALS

Construction: pRS414, GALS promoter

Marker(s):TRP1,ampR

Construct size (kb): 5.428999900817871

Features: marker(s): TRP1
marker(s): ampR
promoter for expression: GALS
MCS: XhoI...SpeI
terminator: CYC1
centromere: CEN6/ARSH4

Applications

YX-type (expression) shuttle vector

vector with low copy number

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--5.4; SacI/XbaI--3.2, 1.8, 0.41.

The galactokinase (GALS) promoter is a deletion variant of GAL1, which lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose.

The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.

Low copy number shuttle expression vector.

One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736