Vector Information
Size (kb): 6.514
DESCRIPTION OF VECTOR:
Intact vector size: 6.514
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS414
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 200-1213
terminator: CYC1, ->, 1895-2155
MCS: XhoI...XbaI, ->, 2155-2219
promoter for expression: ADH, <-, 2219-3719
marker(s): ampR, <-, 4966-5896
replicon: CEN6/ARSH4, ->, 6001-6514
Vector: p414 ADH (plasmid)
Promoters: Promoter for expression ADH
Construction: pRS414
Marker(s):TRP1,ampR
Construct size (kb): 6.514
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: ADH
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
