Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3, 1.8, 0.5; EcoRV--4.2, 1.5; HindIII--4.5, 1.3.
Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736
