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alpha s-Q227L (GSL) rat
alpha s-Q227L (GSL) rat
規格:
價格:
編號:B222076
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
alpha s-Q227L (GSL) rat
商品貨號
B222076
Designations
alpha s-Q227L (GSL) rat
GenBank Number

M12673

培養基
Species
Rattus norvegicus, rat
Depositors
HR Bourne, P Herzmark
Applications
in another host, produces protein guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.600000023841858
Gene product: guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 [GNAS1]
Target Gene: guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1
Insert Size (kb)
1.600
Media
ATCC® Medium 1890: LB medium (ATCC medium 1065) with 12 mcg/ml tetracycline and 100 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.0, 0.6; EcoRI--5.6; HindIII--5.6; HindIII/SalI--4.0, 1.6; SalI--5.6.
Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.4, 0.95; BglII--1.1; EcoRI--0.65; NcoI--0.2; PstI--0.3, 1.0; PvuII--0.3, 0.75.
Deposited with the understanding that the DNAs not be used in human experiments.
The protein produced is the constituitively activated Q227L mutation (glutamine replaced by leucine at codon 227).
Encodes a hemagglutinin epitope replacing nt 475-489 of the GenBank sequence, followed by an altered serine codon (TCC) nt 490-492.
Restriction site(s) at the 3' end of the insert for linearizing the construct: XhoI, NotI, HindIII.
References

Levis MJ, Bourne HR. Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity. J. Cell Biol. 119: 1297-1307, 1992. PubMed: 1280272

Paul Herzmark, personal communication

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