Restriction digests of the clone give the following sizes (kb): SacI/XbaI--4.0, 3.0, 0.4; EcoRI--5.2, 2.3; EcoRV--5.1, 2.4. Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter is repressed when cells are grown in the presence of methionine. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
