| Vector Information |
Size (kb): 7.3670001029968260 DESCRIPTION OF VECTOR:
Intact vector size: 7.367
Type of vector: plasmid
Cloning sites: SalI HindIII PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI
XbaI_
Other unique sites: SacI
Construction: pRS425
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: SalI...XbaI, ->, 3394-3452
promoter for expression: CYC1, <-, 3452-3739
marker(s): ampR, <-, 4870-5800
replicon: 2 micron, ->, 5920-7260 Vector: p425 CYC1 (plasmid) Promoters: Promoter for expression CYC1 Construction: pRS425 Marker(s):LEU2,ampR Construct size (kb): 7.367000102996826 Features: marker(s): LEU2
marker(s): ampR
promoter for expression: CYC1
replicon: 2 micron
MCS: SalI...XbaI
terminator: CYC1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.9, 3.2, 0.35; XbaI--3.9, 3.5; KpnI--6.0, 1.4. Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence. High copy number shuttle expression vector. |
