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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 7.5100002288818360

DESCRIPTION OF VECTOR:
Intact vector size: 7.510
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS425, GALL promoter
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->
terminator: CYC1, ->
MCS: SpeI...XhoI, <-
promoter for expression: GALL, <-
marker(s): ampR, <-
replicon: 2 micron, ->

Vector: p425 GALL (plasmid)

Promoters: Promoter for expression GALL

Construction: pRS425, GALL promoter

Marker(s):LEU2,ampR

Construct size (kb): 7.510000228881836

Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GALL
replicon: 2 micron
MCS: SpeI...XhoI
terminator: CYC1

YX-type (expression) shuttle vector

Restriction digests of the clone give the following sizes (kb): BamHI--7.8; EcoRI--5.4, 2.3; SacI/XbaI--3.9, 3.2.

The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose.

The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.

High copy number shuttle expression vector.

One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.

Temperature: 37.0°C

component of:ATCC 87670