| Vector Information |
Size (kb): 7.5399999618530270 DESCRIPTION OF VECTOR:
Intact vector size: 7.540
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS425, GAL1 promoter
Host range: Candida robustaSaccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: XhoI...SpeI, ->, 3388-3445
promoter for expression: GAL1, <-, 3452-3912
marker(s): ampR, <-, 5063-5993
replicon: 2 micron, ->, 6093-7433 Vector: p425 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS425, GAL1 promoter Marker(s):LEU2,ampR Construct size (kb): 7.539999961853027 Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GAL1
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.9 (doublet); EcoRI--5.1, 2.4; KpnI--5.8, 1.7. It appears that the SacI site at the start of the GAL1 promoter has been lost. Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
